A Role for PI3Ks in Intracellular Replication but Not Phagocytosis of Wild-Type L. pneumophila

<div><p>(A) Phagocytosis by <i>Dictyostelium</i> wild-type Ax3 or Δ<i>PI3K1/2</i> (untreated or treated with 5 μM WM) of GFP-labeled L. pneumophila wild-type (black bars) or a Δ<i>icmT</i> mutant strain (grey bars) was determined by flow cytometry.</p><p>(B) Release of <i>L. pneumophila</i> wild-type (squares) or Δ<i>icmT</i> (circles) into the supernatant of <i>Dictyostelium</i> wild-type (denoted by filled symbols) or Δ<i>PI3K1/2</i> (denoted by open symbols) was quantified by CFUs.</p><p>(C) Release of wild-type <i>L. pneumophila</i> (CFUs) from <i>Dictyostelium</i> wild-type (filled squares denote untreated; filled triangles denote 10 μM LY) or Δ<i>PI3K1/2</i> (open squares denote untreated; open triangles denote LY).</p><p>(D) Quantification by flow cytometry of intracellular growth of GFP-labeled wild-type L. pneumophila within wild-type <i>Dictyostelium</i> or Δ<i>PI3K1/2</i> in the presence or absence of 20 μM LY.</p><p>The data shown are means and standard deviations of duplicates (A) or triplicates (B and C), and are representative of at least three independent experiments (A–D).</p>