Comparative Genomic Analysis of S. japonicum Genes with CDSs from Representative Sequenced Genomes

<div><p>(A) The deduced S. japonicum proteins were compared with public protein datasets from mammalian hosts <i>(Homo sapiens, R. norvegicus,</i> and <i>M. musculus),</i> fish <i>(T. nigroviridis</i> and <i>T. rubripes),</i> insects <i>(A. gambiae</i> and <i>D. melanogaster),</i> nematodes <i>(C. briggsae</i> and <i>C. elegans),</i> protozoans (<i>P. yoelii nigeriensis</i> [17XNL], P. falciparum [3D7]), <i>(Cryptosporidium parvum</i> and <i>C. hominis),</i> and fungi <i>(S. cerevisiae)</i> at a cutoff of different BLASTP E values indicated by icons. The black bars indicated that the numbers of the deduced protein were identified by this proteomic analyses.</p><p>(B) Potential Phylum Platyhelminthes- and <i>Schistosoma</i>-specific genes were predicted through interrogating the platyhelminth and <i>Schistosoma</i> ESTs in GenBank. The numbers within the largest (orange) circle indicate S. japonicum CDSs that were not similar to any known protein in a GenBank dataset, which excluded all flatworm sequences at a cutoff of different BLASTP E values. The numbers within the middle-sized blue and small yellow circles represent S. japonicum CDSs with similarity to the EST data from platyhelminths and schistosomes, respectively, at a cutoff of tBLASTN E value of 10⁻²⁰. The numbers within the smallest green circles indicate proteins identified by the proteomic approach.</p><p>(C) S. japonicum CDSs were compared with EST and cluster datasets of S. mansoni using the tBLASTN program at a cutoff of different E values indicated.</p>