Crossover Distribution in Interval 15 for Three Different Individuals

<div><p>The breakpoint of the crossover was determined by genotyping the SNPs (shown as triangles on top of each graph) using PCR product obtained from individual crossovers. Percentages represent the fraction of crossovers counted between two adjacent informative SNPs and are plotted against the position of the crossover for both type A and the reciprocal type B recombinants. The numbers associated with each bar represent the recombination intensities in cM/Mb (see details in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.0020070#pgen-0020070-sd002" target="_blank">Protocol S2</a>).</p><p>(A) Crossover distribution in interval 15 for an individual based on 36 and 24 recombinants recovered from 66,000 and 44,000 meioses, respectively. Note that this individual has one more informative SNP 50 bp before the end of the interval compared to the other two donors. Only a small fraction of the crossovers occur within these last 50 bps, but the estimated recombination intensity ranges between ~200 to 300 cM/Mb because this region is so small.</p><p>(B) Crossover distribution in a second donor based on 37 and 12 recombinants recovered from 66,000 and 24,000 meioses, respectively.</p><p>(C) Crossover distribution for a third donor based on 23 and 43 recombinants recovered from 66,000 and 88,000 meioses, respectively. For a detailed comparison of the crossover distribution with the three LD based estimates, see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.0020070#pgen-0020070-sd005" target="_blank">Protocol S5</a>.</p>