Telomere Length-Analysis in Outbred Yeast Strains

<div><p>(A) Variation of telomere length among natural isolates of <i>S. cerevisiae.</i> Southern blot with terminal restriction fragments from diploid strains (lanes 1–12, 14) isolated from vineyards, their haploid derivatives (lanes 13, 15–17), and two laboratory strains BY (lane18) and A363A (lane 19). Haploid strains RM5–1 (lane 13) are derived from diploid strain Bb24(4) (lane 9), haploid strain Y55a (lane 15) from diploid strain Y55 (lane 14), haploid strain RM3-1a (lane 16) from diploid strain Bb3(1) (lane 8), and haploid strain RM4–1 (lane 17) from diploid strain Bb24(3) (lane 4). Other diploid strains include Bb26(5) (lane 1), Bb31(2) (lane 2), Bb32 or RM11 (lane 3), PC25A (lane 5), Ba25 (lane 6), Bb2(1) (lane 7), Bb25(6) (lane 10), Bb 25(8) (lane 11), and Bb 26(2) (lane 12).</p><p>(B) Average telomere length-variation in 122 haploid progeny of a cross between vineyard (RM11-1a, a haploid progeny of diploid RM11) and laboratory (BY) strain. Average telomere length of the progeny is continuously distributed between 222 and 342 bp, consistent with the genetic trait controlled by several loci. The average telomere length in the parents, obtained through three independent measurements, was 350 ± 7 and 256 ± 5 for BY and RM11, respectively.</p><p>(C) Genome scan for loci that control telomere length in BY and RM11. The arrows indicate significant loci at Chromosome 12 and Chromosome 13.</p>