Effect of Small Molecules on Wild-Type and Mutant C. elegans

<div><p>(A) The gonad/vulval region of wild-type worms is shown. In the left panel, black arrows indicate the normal, organized array of early stage eggs. The right panel shows a worm treated with BMS-192364 at 0.3 mM. The white arrows indicate late stage eggs that have been retained in the gonad.</p><p>(B) Dose-response curve for BMS-192364, showing effect on egg laying in C. elegans. The percentage of worms displaying an Egl-d phenotype was determined by counting the number of “commas” contained within the animal.</p><p>(C) Quantification of the Egl-d phenotype in four C. elegans mutant strains—<i>ep271, ep272, ep273,</i> and <i>ep275—</i>that were identified in a screen for resistance to the small molecule. Black bars, no treatment. Grey bars, worms treated with BMS-192364 at 0.4 mM.</p><p>(D) Table showing identity of the affected gene in <i>C. elegans–</i>resistant mutant strains, the amino acid changes, and predicted effect on protein function.</p>