Inhibition of Endocytosis or Early Endosome Function Does Not Restore Vpu-Deficient HIV-1 Release

<p>HeLa cells were transfected with NL4.3 (A) or NL4.3delVpu (B) proviral plasmids along with plasmids expressing GFP or Vpu (as controls), dynamin K44A, DN EPS-15, or wild-type and mutant Rab5a proteins. Cell and virus lysates were harvested 48 h post-transfection, and analyzed by Western blotting with an HIV-1 CA-specific monoclonal antibody