DNA Microarray Analysis of Subcellular mRNA Populations

<div><p>(A) Moving average analyses of the fraction of mRNAs encoding MS proteins. Data for two representative fractionations are shown. In each case, well-measured array elements representing characterized genes were extracted, and the local enrichment for MS-encoding genes (window size = 151) was calculated as a function of the Cy5/Cy3 ratio. The horizontal line represents the overall fraction of MS genes on the microarrays used in these experiments.</p><p>(B) Discovery rate analysis for the identification of MS and CN genes. A representative microarray hybridization was chosen for each cell line and the total nonredundant number of classified MS or CN genes (UniGene clusters) was calculated after each new fractionation.</p>