Nicotinamide Inhibits Ethanol-Induced Neurodegeneration

<div><p>(A) Fluoro-Jade B staining 24 h after ethanol treatments.</p> <p>(B) Quantification of Fluoro-Jade–B positive cells in the cingulate cortex (ANOVA <i>F_2,13</i> = 25.541; <i>p <</i> 0.0001; <i>n</i> = 5–6 each treatment group), in the total CA1 region of hippocampus (ANOVA <i>F_2,13</i> = 9.988; <i>p</i> = 0.0024; <i>n</i> = 5–6 each treatment group), and in the LDN of thalamus (ANOVA <i>F_2,13</i> = 20.785; <i>p</i> < 0.0001; <i>n</i> = 5–6 each treatment group). </p> <p>(C) Total number of NeuN positive cells were counted 2 wk after ethanol administration in the cingulate cortex (ANOVA <i>F_2,12</i> = 9.894; <i>p</i> = 0.0029; <i>n</i> = 5 each treatment group), in the CA1 region of hippocampus (ANOVA <i>F_2,12</i> = 7.289; <i>p</i> = 0.0085; <i>n</i> = 5 each treatment group) and in the LDN of thalamus (ANOVA <i>F_2,12</i> = 5.551; <i>p</i> = 0.0196; <i>n</i> = 5 each treatment group). Values are shown as mean ± SD. Bonferroni correction for multiple comparison revealed a significant difference between ethanol treatment group and all other groups. </p> <p>S, saline; E, ethanol; Etoh+Nic, ethanol + nicotinamide. Scale bars 200μm.</p>