Folding Free Energies of 5′-UTRs

<p>(A) Cumulative distributions of folding free energies, Δ<i>G,</i> are shown for 5,888 ORFs for 5′-UTRs (50 nt upstream of the ORF; solid line), 3′-UTRs (50 nt downstream of the ORF; dashed-dotted line), coding sequences (50-nt sequences following downstream of the start codon of each ORF; dotted line), and 5,888 sequences of length 50 nt selected randomly from intergenic regions (dashed line).</p> <p>(B) Distribution of <i>Z</i>-scores for 5′-UTRs of 5,888 ORFs. Each 5′-UTR sequence was shuffled 100 times and a <i>Z</i>-score was calculated for each to compare the folding free energy of the native sequence to the shuffled sequences. A histogram of these <i>Z</i>-scores is shown together with a standard normal distribution (dashed line).