RNA Synthesis Phenotype of MHV-A59 <i>ts</i> Mutants

<p>RNA synthesis was determined using a 1 h pulse label with ³H-uridine in the presence of dactinomycin and cycloheximide, given to wt MHV-A59 and <i>ts</i> mutant virus-infected cells at 8 hpi with or without shifting from the permissive to the non-permissive temperature. The amount of incorporated ³H-uridine at 40 °C was divided by that at 30 °C and 1.0 was subtracted. The results represent the average of five separate experiments. A value of zero means the incorporation at the two temperatures was the same.