Single cycle replication kinetics and plaque morphologies using WT and re-encoded viruses.

<p>To study replicative fitness of the re-encoded viruses, all viruses (including the WT) were used to perform single cycle replication kinetics in Vero (<b>A–B</b>) HEK293 (<b>C–D</b>) and C6/36 cells (<b>E–F</b>). An estimated MOI of 5 was used to infect confluent cells. Cells were washed 30 minutes after the infection and 1 ml of cell supernatant was sampled at several time pi. Clarified (centrifugation) cell supernatants were used to measure (i) the infectious titres using a TCID50 assay (<b>A, C, E</b>) and (ii) the amount of viral RNA using a real time RT-PCR assay (<b>B, D, F</b>). The plaque morphology of these viruses was also analysed comparatively in Vero cells (<b>G</b>).