Quantitative Measurements of Flow Changes in Cortical Arterioles after Filament Occlusion of the MCA

<div><p>(A) Example of flow changes observed following MCA occlusion. Left: projection of a TPLSM image stack taken at baseline. Center: tracing of the surface arteriole network from the image with the baseline blood flow speed and direction indicated in some vessels. Right: blood flow speed and direction during MCA occlusion. Red arrows and speed labels indicate vessels whose direction has reversed. The axes indicate the rostral (R) and medial (M) directions.</p> <p>(B–D) RBC speed, vessel diameter, and volume blood flux, respectively, during MCA occlusion as a function of baseline values. The baseline and occlusion values of the diameter and flux are significantly correlated (<i>p</i> < 0.005), with r² = 0.92 and 0.26, respectively; the baseline and occlusion values of the speed are not significantly correlated.</p>