Rescue of Visual Responses Measured by Single-Cell Recording and ERG Responses of single <i>Lrat</i>^+/+ and <i>Lrat</i>^−/− Rods

<div><p>(A–D) Flash families measured for a <i>Lrat</i>^+/+ rod (A), a control <i>Lrat</i>^−/− rod (B), a <i>Lrat</i>^−/− rod after a single gavage with 9-<i>cis</i>-R-Ac (C), and a <i>Lrat</i>^−/− rod after three gavages with 9-<i>cis</i>-R-Ac (D). Each panel superimposes average responses to five to 20 repeats of a flash, with the flash strength increasing by a factor of two for each successively larger response.</p> <p>(E) Stimulus-response relations for the same cells in (A–D). Maximal response amplitudes are plotted against the flash strength. Fits are saturating exponential functions, used to estimate the strength of the flash producing a half-maximal response (<i>Lrat</i>^+/+, 26 photons/μm²; <i>Lrat</i>^−/−, 43,000 photons/μm²; singly treated <i>Lrat</i>^−/−, 590 photons/μm²; and multiply treated <i>Lrat</i>^−/−, 69 photons/μm²).</p> <p>(F and G) Comparison of WT mice scotopic single flash ERG to <i>Lrat</i>^−/− 9-<i>cis</i>-R-Ac gavaged mice and <i>Lrat</i>^−/− and <i>Lrat</i>^+/+ control mice. <i>Lrat</i>^−/− mice were gavaged nine times with 5 μmol 9-<i>cis</i>-R-Ac over a 1-mo time period (<i>n</i> ≥ 3, data shown with SD).</p>