Mean Biological Effect of Antibodies in Either Direct or Monocyte-Dependent Fashion, at Various Time Points with Each Adjuvant

<p>Shown are the means ± standard error of the mean of the effects of sera from all volunteers in direct growth inhibition assays (used as a control in each ADCI assay; see Methods) and in monocyte-dependent ADCI assays (sera from 30 volunteers were analyzed at each time point, i.e., the figure summarizes results from 90 sera). Triangles, Montanide-adjuvated vaccine; circles, alum-adjuvated vaccine. Open symbols, direct growth inhibition by antibodies; solid symbols, monocyte-dependent ADCI assays. Months 0, 5, and 12: sera collected before immunisation, 1 mo after the last immunisation, and 12 mo after the first immunisation, respectively.