Schematic illustration of the experimental scheme for the prediction of osteogenic differentiation potential using multiple and time-course morphological features.

<p>hBMSCs were cultured in non-induction medium in first 4 days, then the medium was replaced with osteogenic induction medium only for the Induction sample. From day 0 to day 14, cell images were automatically acquired by BioStation CT every 8 hours. ALP activity and calcium deposition rates were evaluated on days 14 and 21, respectively. Using multiple morphological features covering 2 weeks culture, two types of hBMSC osteogenic differentiation evaluation results were predicted by individual prediction models.