SsrA-Tagging Variants Complement the ΔBA Mutant to Different Degrees

<div><p>(A) Cultures of wild-type (WT) or the ΔBA mutant strains were grown in low-calcium medium at 37 °C. Bacteria were removed by centrifugation and the secreted proteins were precipitated with TCA, resolved by electrophoresis on a 10%-polyacrylamide Tris-tricine gel, and stained with Coomassie Brilliant Blue.</p><p>(B) To measure motility, equal numbers of bacteria from each strain were inoculated onto 0.25% agar-T medium plates and images were obtained after incubation for 48 h at room temperature.</p><p>MW stds, protein molecular weight standards.</p>