In Vitro Oocyst Sporozoite Release Assay

<p>Release of oocyst sporozoites at 25 °C in vitro in the presence of trypsin (50 μg/ml) at day 14 PI. <i>Y</i>-axis represents released sporozoites as a percentage of total oocyst sporozoites. <i>X</i>-axis indicates the time point when the samples are collected. In the absence of the trypsin, very few sporozoites are released from the midguts. In the presence of the trypsin, oocyst sporozoites from both CS-WT (WT) and CS-RIImut (RII) are released in a time-dependent manner. Compared with CS-WT, CS-RIImut sporozoites are released more slowly.