Ang-2 Alone Disrupts Endothelial Architecture at Physiologic Concentrations

<div><p>(A) Control (vehicle) or recombinant human Ang-2 (100 ng/ml) was added to HMVEC monolayers. These cells were then fixed and stained for F-actin and VE-cadherin. Shown are healthy control cells (panels a–c) versus Ang-2 treated cells (panels d–f), which exhibit thick actin stress fibers and disrupted junctions, leaving intercellular gaps (arrows).</p> <p>(B) HMVECs were grown to confluence on Transwell membranes coated with fibronectin. Monolayers were treated with vehicle or Ang-2 (400 ng/ml in luminal chamber) plus FITC-albumin. P_a was calculated after 8 h as described in the Methods section. P_a values are expressed as percentage of control cells.*<i>p</i> < 0.01.</p>