The Dauer-Constitutive Phenotype of <i>daf-2(e1370)</i> Is Enhanced by Many RNAi Clones That Extend Lifespan in a <i>daf-16-</i>Dependent Fashion

<p>Relative dauer formation of <i>daf-2(e1370)</i> animals grown at 22.5 °C on RNAi clones versus vector control is shown, average of two to three experiments. 30–50% of the animals on vector control become dauers at 22.5 °C. Total number of dauers/total number of animals observed is noted on top of bars. Error bars: ± SEM. ‘*', previously characterized RNAi clones [<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.0010017#pgen-0010017-b09" target="_blank">9</a>] served as negative (<i>daf-16,</i> RNAi insert consists of first 1.2 kb cDNA) and positive (<i>daf-2,</i> RNAi insert consists of first 2.2 kb cDNA; see also <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.0010017#pgen-0010017-sg002" target="_blank">Figure S2</a>) controls for the dauer experiment. <i>daf-2(e1370)</i> worms grown on <i>ddl-3</i> RNAi gave rise to almost no progeny; therefore, this <i>daf-16</i>-dependent RNAi clone was not assayed.