Immunohistochemical Localization of CEACAM1, CSNK1D, and PRKCB1 in Colonic Mucosa

<div><p>Staining of a representative mucosal tissue samples from five normal controls (N), five Crohn disease (CD), and six ulcerative colitis (UC) patients using antibodies against (A) CEACAM1, (B) CSNK1D, and (C) PRKCB1.</p> <p>(A) CEACAM1 immunoreactivity was found in the apical epithelial lining (1), crypts of inflamed tissue (2, 4). Additional staining was detected in immune cells (3) and blood vessels (5).</p> <p>(B) CSNK1D immunoreactivity showed a strong granular staining pattern in normal (6) and the UC group (8), which was located basolaterally. Weaker staining of the apex of the crypts could be detected in CD (7).</p> <p>(C) For PRKCB1, weak staining could be observed in the apical epithelial layer in the normal (9) and CD (10) mucosa and, interestingly, immunoreactivity was found nearly exclusively in the marginal zone of small lymph follicles (11), whereas the lamina propria was immunonegative. In contrast, strong staining was detected in the apical epithelial layer of UC mucosa (12). Furthermore, lamina propria mononuclear cells underlying the epithelial layer were also positive in the UC mucosa (13).</p>