IIGP1-Positive Vesicular Structures Are Located at Sites Where the PVM Is Disrupted

<p>Astrocytes were induced with IFN-γ<i>,</i> infected with <i>T. gondii</i> and fixed 6 h later. (A–C) Ultra- thin cryosections were labeled for IIGP1 using the 165 antiserum and 10 nm gold coupled to protein A. The insets in (A) show enlarged views of the boxed regions. The black arrows in the bottom left inset point to IIGP1-labeled vesicular profiles with an apparent electron-dense coat. (D and E) Astrocytes were labeled with the anti-GRA7 mAb and 10 nm gold coupled to protein A. (open white arrowhead: <i>T. gondii</i> inner membrane complex [IMC]; filled white arrowhead: <i>T. gondii</i> plasma membrane; black arrowhead: PVM). Bars: 250 nm.