Induction of Autophagosomes in Vicinity of Disrupted Vacuoles

<p>Astrocytes were transfected with pEGFP-C3-LC3 and induced with IFN-γ<i>.</i> Cells were infected with <i>T. gondi</i> 24 h later and fixed after 2 h (A) or 6 h (B, C, and D). In cells containing only smooth IIGP1 vacuoles GFP-LC3 remained diffusely distributed throughout the cytoplasm (A). In cells containing disrupted IIGP1 PVs GFP-LC3 localizes to vesicular and filamentous structures that are in close proximity to, but do not engulf the IIGP1-positive PVs (B and C). The arrowheads point to IIGP1-positive PVs. The images shown in (A) were processed by 2D deconvolution. (D) Shows maximum projections of 3D deconvoluted Z-series.