Distribution of GFP-Expressing Endothelia and Kupffer Cells in the Liver

<div><p>(A) Confocal microscopy demonstrates the GFP distribution in sinusoidal endothelia from a Tie2-GFP mouse. GFP is most prominent in the perinuclear region (arrowheads) of endothelia located in the periphery of the liver lobule.</p> <p>(B) A still image from an intravital movie shows GFP-expressing endothelia lining the sinusoids of a Tie2-GFP mouse. Kupffer cells can be identified by their orange autofluorescent lysosomes (arrowheads).</p> <p>(C) Star-shaped Kupffer cells (arrowheads) are located in sinusoids of a lys-EGFP-ki mouse liver.</p> <p>(D) Round blood granulocytes (arrows), traveling with the bloodstream or crawling along the sinusoidal cell layer, exhibit a stronger GFP signal than Kupffer cells (still image extracted from an intravital movie). Note the orange autofluorescence of the Kupffer cell lysosomes (arrowheads).</p> <p>Bar = 10 μm. See <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.0030192#sv001" target="_blank">Videos S1</a> and <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.0030192#sv002" target="_blank">S2</a>.</p>