Human ES Cell or Foetal-Derived NS Cells

<div><p>(A) Derivation from human ES cells: human ES cell primary culture (a), differentiation of human ES cells into neural-rosette structures (b), passage 9 in NS expansion medium (c), individual cells exhibit radial glial morphology (d), and immunostaining for NS cell/radial glia markers (e–h).</p> <p>(B) Derivation from human foetal forebrain: floating clusters (i) generated from cortex, attachment and outgrowth (j), passage 5 in NS expansion medium (k), radial glia morphology (l), and NS cell/radial glial markers (m–p).</p> <p>(C) Differentiation of human foetal NS cells: TuJ1 positive neuronal cells generated by sequential growth factor withdrawal (q), and GFAP positive astrocytes induced by exposure to serum (r).</p>