TrkA/TrkC and PV Immunohistochemistry in DRG and Spinal Cord

<div><p>Red represents TrkA, green represents TrkC (and PV in [C]), and yellow represents co-expression.</p> <p>(A) TrkA/TrkC immunostaining in E15 DRG. TrkC-positive neurons normally eliminated in <i>NT-3</i> KOs are rescued in double KOs.</p> <p>(B) TrkA/TrkC immunostaining at P0 in DRG.</p> <p>(C) PV immunostaining in P0 DRG. Rescued TrkC-positive cells fail to express PV.</p> <p>(D) Ratio of TrkA-immunopositive cells to TrkC-immunopositive cells in E15 and P0 DRGs. Data are presented as percentage of cells with standard deviation. Double KOs always had similar ratios to <i>Bax</i> KOs, and <i>NT-3</i> KOs had the least amount of TrkC-positive cells, if any.</p> <p>(E) TrkA/TrkC immunostaining in E15 spinal cord. Arrow points to group Ia fibers. Dorsal is up.</p> <p>Scale bar: 50 μm (A–C), 1 mm (E).</p>