Mcd1p Binding within the Endogenous CHRIII Pericentric Region after Centromere Excision or Centromere Movement

<p>The Mcd1p binding profiles in the endogenous CHRIII pericentric region are shown in cells in which the centromere is absent, either because of centromere excision (gray circles, PMY185) or because of the movement of the centromere to an ectopic location on the right arm of CHRIII (black triangles, PMY318). PMY185 and PMY318 are highly related strains; PMY185 was one of the parental strains used to generate PMY318. In the centromere excision strain, Mcd1p binding was examined in the same cell cycle in which the centromere was lost, whereas in the ectopic centromere strain, Mcd1p association was determined many generations after centromere relocation (see text for further discussion). Mcd1p binding data from the centromere excision experiment are the same as those shown in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.0020260#pbio-0020260-g002" target="_blank">Figure 2</a>B, but are replotted here for clarity. <i>CEN3</i> normally occupies the interval between SGD coordinates 114382-114498.