Effect of the −243 A>G , −1600 G>A, and −2004 A>T SNPs on Transcriptional Activity

<p>G, A, and T alleles at −243 A>G, −1600 G>A, and −2004 A>T SNPs were generated into the wild-type construct (wt). βTC3 cells were transfected with equivalent amounts of pGL3, wild-type, Gad-2004T, Gad-1600A, and Gad-243G constructs. pRLTK was cotransfected with each construct. Luciferase and <i>Renilla</i> activities were assayed and normalized. Each experiment was performed in duplicate and replicated five times. A 6-fold increase of transcriptional activity was observed for the Gad-243 as compared to the wild-type construct.