CD8⁺ T-Cell Differentiation and Senescence

<div><p>(A) Expression of the replicative senescence-associated marker CD57 on antigen-experienced CD8⁺ T-cell subsets. The percentage and mean fluorescence intensity for the CD57⁺ cells are shown for one single donor. Data on several donors (HIV-1-infected or healthy) are also shown (<i>n</i> = 24).</p> <p>(B) Expression of CD57 on CD8⁺ T-cells (whole population or antigen-specific) from acute to postacute (on ART) HIV-1 infection.</p> <p>(C) CD69 expression and CFSE proliferation profile for CD8⁺ T-cell subsets gated on the basis of CD57 and CD27 expression following stimulation with anti-CD3 antibodies. PBMCs were analysed for CD69 expression after 18 h and CFSE labeling after 6 d. Percentages of proliferating cells (with background subtracted) are indicated. Representative results from three experiments (one HIV-infected and two healthy donors) are shown.</p> <p>(D) Telomere length measurement by flow FISH on naïve and antigen-experienced CD8⁺ T-cell subsets FACS-sorted on the basis of CD57, CD27, CCR7, and CD45RA expression. The average length of telomeres was obtained by substracting the mean fluorescence of the background control (no probe; open histogram) from the mean fluorescence obtained from cells hybridised with the FITC-labeled telomere probe (gray histogram). Representative results from two experiments (on healthy donors) are shown.</p> <p>(E) CD57 and perforin expression in the CD8⁺ T-cell population dissected into naïve (CD27^+high, perforin-negative), antigen-experienced CD27⁺ (perforin^low), and antigen-experienced CD27⁻ perforin^low or perforin^high subsets. The percentage and mean fluorescence intensity for the CD57⁺ cells are indicated.</p> <p>(F) Representative staining for perforin and CD57 in CD8⁺ T-cells from a HIV-1-infected or a healthy donor. Percentages of cells present in the top quadrants are shown.</p> <p>(G) Representative staining for perforin and CD57 in CD4⁺ T-cells from an HIV-1-infected or a healthy donor. Percentages of cells present in the top quadrants are shown.</p>