Phenotypic assessment of transformed cells.

<p>Clones from p21 cells were assessed for ductulogenesis (A), invasiveness (B) and seeding phenotypes (C). <b>A</b>) Ductulogenic assay was conducted on the collagen matrix. <b>B)</b> Invasive characteristic of clone was compared to MDA-MB 231 cells using invasion assay in BD BioCoat™ Matrigel™ Invasion Chambers. Histogram of invaded cells expressed as mean invaded cells per field. <b>C</b>) Phenotype of transformed clone (MCF-10AT) and wild type MCF-10A cells were captured through acquiring images of these cells after 3hrs and 24 hrs of seeding in a monlayer culture using an Olympus C-5060 digital camera attached to the Nikon TE2000U inverted microscope with 20x and 40x objectives.