The growth of 4-OH-E2 transformed MCF-10AT clone was stimulated by 17 beta-estradiol (E2) and inhibited by both antioxidants, Ebselen and N-acetyl cysteine.

<p>Cells were grown in 96-well plates for 2 days in 10% FBS DMEM/F12 and serum starved 2 days prior to addition of E2 (100 pg/ml) for 18 h-48 h unless specified otherwise. Bromodeoxy uridine (BrdU) incorporation assay was used to measure DNA synthesis in transformed cells. Antioxidants ebselen, NAC, and catalase were pretreated for 2 h prior to the addition of E2. Colorimetric BrdUrd incorporation was measured at 450 nm with a plate reader. ROS production was measured by 10 uM H2DCFDA oxidation on a 96 well plate reader. Results are expressed as mean ± SD of three separate experiments with control set as 100%. *P<0.05, significantly different from control. **P<0.05 indicates significantly different from E2, (P<0.05).