Effect of MAP65-1 on the dynamics of individual MTs.

<p>(<b>A</b>)<b>.</b> Kymographs of single MTs that elongate in the absence of MAP65-1 (left kymograph), or in the presence of 0.1 µM of GFP-MAP65-1 (right kymograph). In the first kymograph, a single Alexa-488 MT (green) elongates from an Alexa-568 MT seed (red). In the right kymograph, Alexa-568 MT (red) elongates from an Alexa-568 MT seed (red) in the presence of GFP-MAP65-1 (green). The yellow color reveals the binding of GFP-MAP65-1 on the MT. Schemes on top of the kymographs show the orientation of MT ends. Bottom images show the MTs used to draw the kymographs. (<b>B</b>)<b>.</b> Length history plots of 3 single MTs over time in the absence and in the presence of 0.1 µM MAP65-1. (<b>C–D</b>)<b>.</b> Distribution of elongation rates (C) and shortening rates (D) of single MTs in the absence (top row) or in the presence of 0.1 µM MAP65-1 (bottom row). Data for MT (+) and (−) ends are shown in blue and green respectively. Average rates and population size are indicated. (<b>E</b>)<b>.</b> Duration of elongation phases of single MTs after a rescue event in the absence and in the presence of 0.1 µM MAP65-1. Black dots indicate the mean duration of elongation phases. (<b>F–G</b>)<b>.</b> Depolymerization length of MTs in the absence and in the presence of 0.1 µM MAP65-1, for MT minus end (F) and MT plus end (G).