Cell proliferation and viability after primary infection of B cells.

<p>B cells isolated using anti-CD19 magnetic beads (<b>A and B</b>) or ficoll-purified bulk lymphocytes from a buffy-coat residue (from donor D11) (<b>C and D</b>) were infected by the viruses indicated, at day zero. The proportion of proliferating cells (<b>A and C</b>) was assessed by EdU incorporation over 16 hours. Cell viability was assessed by Live/Dead staining (<b>B and D</b>). The sampling time after infection, and the virus used to infect the cells are as indicated. Note that FACS statistics were not collected for 3CHT+HT at 27 days due to a technical failure during automated data acquisition (_*). However visual inspection indicated that the majority of the cells were viable and this was consistent with the yield of RNA recovered from these cells (which was comparable to the WT and revertant infections – see <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003187#ppat-1003187-g007" target="_blank">figure 7</a>).