The CIRBP-dependent UVC-induced accumulation of IL1B, IL8 and TNFAIP6 mRNAs.

<p>(A) Fibroblasts were transfected with either control (NT), CIRBP or IL1B siRNAs, 72 hours later cells were exposed to either 0 or 20 J/m² of UVC light and total RNA was collected at the indicated time following UVC exposure for qRT-PCR analysis of the indicated mRNAs. (B) Fibroblasts were similarly transfected with siRNAs 72 hours prior to UV exposure Cells were collected by scraping and IL-1β protein was measured by ELISA at 16 hours following UVC exposure. (C) Cells were transfected with the indicated expression vector and 24 hours later cells were collected and analyzed as described in (B). Each value in A, B and C represents the mean (± SEM) determined from a minimum of 3 independent experiments. An * indicates that the value is significantly different than similarly treated control samples (P≤0.05, t test) while a # indicates that the value is significantly different from 1.0 (P≤0.05, single sample t-test).