Flow cytometric analysis of immune cell composition in liver after alcohol treatment.

<p>(A) Representative histograms of liver mononuclear cells (LMNCs) in wild type B6 mice (upper panel) and IRAK-M−/− mice (lower panel). (B) Summary of immune cell composition of LMNC in wild type B6 (blue) and IRAK-M−/− mice (red) in ALC groups. (C) Summary of immune cell composition of LMNC in wild type B6 (blue) and IRAK-M−/− mice (red) in CTL groups. (D) Representative dot plots of Treg (CD4⁺Foxp3⁺) cells in total LMNCs of control (CTL, left panel) and binge alcohol treated (ALC, right panel) WT B6 (upper panel) and IRAK-M−/− mice (lower panel). (E) Summary of percentage of Treg cells in total LMNCs of CTL and ALC treated WT B6 (blue) and IRAK-M−/− B6 (red) mice. (F) Representative dot plots of Treg (CD4⁺Foxp3⁺) cells in total splenocytes of control (CTL, left panel) and alcohol treated (ALC, right panel) WT B6 (upper panel) and IRAK-M−/− mice (lower panel). (G) Summary of percentage of Treg cells in total splenocytes of CTL and ALC treated WT B6 (blue) and IRAK-M−/− B6 (red) mice. Error bars represent the <i>SD</i> of samples within a group. The experiment was performed 3 times and the data presented are from one of the 3 experiments. **<i>P</i><0.01, Two way ANOVA analysis. <i>N.S.</i> not statistically significant.