Altered gut permeability and composition of gut bacteria in the intestine after alcohol treatment.

<p>(A) FITC-dextran concentration in blood after gut permeability test in wild type B6 mice (blue) and IRAK-M−/− mice (red). (B) LPS content in the blood of B6 mice (open bar) and IRAK-M−/− mice (solid bar). (C) Number of culturable bacteria in the intestine before (blue) and after (red) binge alcohol treatment (ALC) in wild type B6 and IRAK-M−/− mice. (D) G⁺/G⁻ gut bacteria ratio from mouse feces tested by Q-PCR before (blue) and after (red) binge alcohol treatment in wild type B6 and IRAK-M−/− mice. Experiments were performed 3 times for A and twice for B, C and D. The data presented in A, C and D were from one of the experiments, and those shown in B were from pooled 2 experiments. n = 2–3 in each group of each experiment. Error bars represent the <i>SD</i> of samples within a group. *<i>P</i><0.05, **<i>P</i><0.01 (Student’s t-test).