Insertion of a retrotransposon in the <i>Sd</i> mouse.

<p>A. Structure of the wild-type and ETn alleles. The 8,497-bp of ETnERV3 was inserted 1,418-bp upstream of the first exon of <i>Gm13344</i> and 8,114-bp downstream of the last exon of <i>Gm13336</i>. Each box indicates an exon. Arrows indicate the direction of transcription of the <i>Gm13344</i>, <i>Gm13336</i>, and <i>Ptf1a</i> genes. Black bold bars indicate genomic probes used for Southern blotting. “S” indicates Sph I restriction sites. Bold arrows with numbers indicate primers used for PCR analyses. The red arrowhead indicates the insertion point of the ETn. B. Genotyping by Southern blotting. An approximately 9.4-kb band or 18-kb band corresponded to the wild-type (WT) and ETn alleles, respectively. C. Genotyping by genomic PCR analysis. Primer pairs 1 and 2 or 1 and 3 were used to detect the WT or ETn alleles, respectively.