Phenotypic rescue by disruption of the <i>Gm13336-Ptf1a</i> gene.
- Kei Semba (380996)
- Kimi Araki (380997)
- Ken-ichirou Matsumoto (380998)
- Hiroko Suda (380999)
- Takashi Ando (381000)
- Akira Sei (381001)
- Hiroshi Mizuta (381002)
- Katsumasa Takagi (381003)
- Mai Nakahara (381004)
- Mayumi Muta (381005)
- Gen Yamada (134986)
- Naomi Nakagata (134967)
- Aritoshi Iida (381006)
- Shiro Ikegawa (106723)
- Yusuke Nakamura (25445)
- Masatake Araki (381007)
- Kuniya Abe (64132)
- Ken-ichi Yamamura (14117)
DOIAbstract
<p>A. Strategy for disruption of the <i>Gm13336-Ptf1a</i> gene. The <i>Gm13336-Ptf1a</i> gene was disrupted using a targeting vector that contained a neomycin resistance (<i>neo</i>) gene flanked by <i>loxP</i> and <i>lox2272</i>. Exons of the <i>Gm13336</i> and <i>Ptf1a</i> genes are shown as white and gray boxes, respectively. B. RT–PCR analyses of E9.5 embryos. Expression of <i>Gm13336</i>, mutant <i>Gm13336</i>, and <i>Ptf1a</i> was not detected in ETn-<i>Gm13336-Ptf1a</i><sup>neo</sup>/ETn-<i>Gm13336-Ptf1a</i><sup>neo</sup> embryos. C. Tail phenotype of E18.5 embryos. Both ETn-<i>Gm13336-Ptf1a</i><sup>neo</sup>/+-+ and ETn-<i>Gm13336-Ptf1a</i><sup>neo</sup>/ETn-<i>Gm13336-Ptf1a</i><sup>neo</sup> embryos showed normal tails, although th...
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