Importance of the R-like recognition sequence for mitochondrial targeting and processing.

<p>(<b>A</b>) Live cell epiflorescence imaging indicates that NTS–ΔRS and NTS–R105A are targeted to mitochondria, while NTS ΔI2–ΔRS and NTS ΔI2–R105A show less efficient mitochondrial targeting in <i>D. discoideum</i>. Scale bars, 10 µm. (<b>B</b>) Immuno-blot loaded with whole cell lysate from untransformed <i>D. discoideum</i> cells (AX2), cells producing EYFP, and EYFP-tagged constructs NTS, NTS–ΔRS, NTS–R105A, NTS ΔI2, NTS ΔI2–ΔRS, and NTS ΔI2–R105A. Similar to NTS, NTS–ΔRS and NTS–R105A undergo processing. Processing is greatly impaired in the case of NTS ΔI2–ΔRS and NTS ΔI2–R105A.