Curcumin potentiates TRAIL sensitivity through induction of ROS.

<p>A, Immunoblots of 1507.2 cells treated with curcumin show reduced levels of p-AKT, p-Stat3 and p-IKBa. B, Curcumin induced ROS in 1507.2 cells dose dependently which was blocked by ROS-scavenger N-acetylcystein (NAC). C, H₂O₂ increased levels of ROS in 1507.2 cells dose dependently. D, NAC rescued cell viability of TRAIL-treated 1507.2 cells dose dependently. E, NAC abrogated the curcumin-induced enhancement of PARP-cleavage. F, H₂O₂ (400 µM) increased the sensitivity of 1507.2 cells to TRAIL. G, H₂O₂ increased PARP cleavage of TRAIL treated 1507.2 cells dose dependently. Results are expressed as mean ± s.d. of three independent experiments (n = 3). Asterisks outline values that are different from respective control (* = P<0.05; ** = P<0.01, Student's t-test).