Effects of tanshinone IIA on PASMCs proliferation under normoxic and hypoxic conditions.

<p>(A–B) Effects of tanshinone IIA on the proliferation of PASMCs under normoxic (21% O₂) and hypoxic (3% O₂) conditions. Hypoxia for 24 h significantly promoted PASMCs proliferation. Tanshinone IIA significantly inhibited hypoxia-induced PASMCs proliferation. At 3 and 10 µg/mL, tanshinone IIA only inhibited PASMCs proliferation under hypoxic condition, but at 30 and 50 µg/mL Tanshinone IIA inhibited PASMCs proliferation under both hypoxic and normoxic conditions (<i>*P</i><0.05 vs normoxic plus 0 µg/mL tanshinone IIA group, <i>^#P</i><0.05 vs hypoxia plus 0 µg/mL tanshinone IIA group). (C) Time course experiments showed that hypoxia for 24 h reached maxmal stimulation on PASMCs proliferation and tanshinone IIA reached maximal inhibition after 24 h incubation (<i>*P</i><0.05 vs corresponding time points in hypoxia group). (D) Effect of tanshinone IIA on PASMCs viability. Tanshinone IIA (3–50 µg/mL) had no significant effects on PASMCs viability under both normoxic and hypoxic conditions. Data are mean ± SEM from three replicate experiments. In this and the following figures, STS represents tanshinone IIA (sodium sulphonate).