PRC2, PhoRC, and PRC1 show different replication timing distributions in <i>Drosophila</i> S2 cells.

<p>(A) Boxplot (left panel) of genome-wide <i>Drosophila</i> embryonic S2 cell line replication timing for significantly H3K27me3 enriched promoters (orange) and non-enriched promoters (light blue). Horizontal boxplot (top right panel) of RNA expression levels in the two previous groups, where group sizes are indicated to the left. Bootstrap distributions (bottom right panel) of mean replication timing for H3K27me3 significantly enriched promoters (orange) and non-enriched promoters (light blue). Percentile confidence intervals (α = 0.025) are indicated with dashed vertical lines. (B) Same as (A), with promoters classified as E(z) bound (orange) and non-bound (light blue). (C) Same as the first two panels of (B), with promoters classified as PHO bound (orange) and non-bound (light blue). (D) Horizontal boxplot of RNA expression levels for genome-wide PRC1 bound (orange) and non-bound (light blue) promoters, PRC1 bound (dark red) and non-bound (dark blue) OFF promoters only and PRC1 bound (red) and non-bound (blue) ON promoters only (see Material and Methods). Group sizes are indicated to the left. (E) Boxplot of replication timing for the three previous groups. (F) Bootstrap distributions of mean replication timing for PRC1 bound (red) and non-bound (blue) ON promoters. Percentile confidence intervals (α = 0.025) are indicated with dashed vertical lines. All p-values have been computed using a Wilcoxon rank sum test.