Quantitative RT–PCR analysis of representative flowering-related genes and <i>Ehd4</i> in various flowering-time mutants or their NILs (near-isogenic lines) and corresponding WTs under SDs and LDs.

<p>(<i>A</i>) Transcript level of <i>Ehd2</i>, <i>Ehd3</i>, <i>OsMADS50</i>, <i>OsGI</i>, <i>OsMADS51</i>, <i>OsphyB</i>, <i>OsCOL4</i> and <i>DTH8</i> in WT (Kita-ake) and <i>ehd4</i> plants. (<i>B</i>) Transcript level of <i>Ghd7</i>, <i>Hd3a</i>, <i>RFT1</i>, <i>Ehd1</i> and <i>Hd1</i> in WT (Nipponbare) and <i>ehd4</i>-<i>Nip</i> plants. (<i>C</i>) Transcript level of <i>Ehd4</i> in various flowering-time mutants or their NILs (near-isogenic lines) and corresponding WTs. Dongjin and the <i>osphyb</i> mutant <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003281#pgen.1003281-Lee1" target="_blank">[26]</a>; Tohoku IL9 and the <i>ehd2</i> mutant <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003281#pgen.1003281-Matsubara1" target="_blank">[34]</a>; Tohoku IL9 and the <i>ehd3</i> mutant <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003281#pgen.1003281-Matsubara2" target="_blank">[36]</a>; Dongjin and the <i>osmads50</i> mutant <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003281#pgen.1003281-Lee2" target="_blank">[31]</a>; Dongjin and the <i>osmads51</i> mutant <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003281#pgen.1003281-Kim1" target="_blank">[30]</a>; Nipponbare and a NIL carrying a non-functional <i>Hd1</i> allele <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003281#pgen.1003281-Yano1" target="_blank">[19]</a>; <u>Aso</u>minori and a NIL carrying a nonfunctional <i>DTH8</i> allele <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003281#pgen.1003281-Wei1" target="_blank">[29]</a>; A NIL carrying a functional <i>Ghd7</i> allele and a NIL carrying a non-functional in the Shanyou 63 background <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003281#pgen.1003281-Xue1" target="_blank">[28]</a>. Taichun 65 carrying a non-functional <i>Ehd1</i> allele and a NIL carrying a functional <i>Ehd1</i> allele <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003281#pgen.1003281-Doi1" target="_blank">[24]</a>; Nipponbare carrying a partially functional <i>Hd3a</i> allele and a NIL carrying a functional <i>Hd3a</i> allele <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003281#pgen.1003281-Kojima1" target="_blank">[20]</a>; Penultimate leaves were harvested around reported peak expression level of each gene during the 24 hrs photoperiod - at dawn for <i>OsphyB</i>, <i>OsCOL4</i>, <i>Ehd1</i>, <i>Ehd2</i>, <i>Hd3a</i>, <i>RFT1</i> and <i>Ehd4</i>, 3 h after dawn for <i>Ghd7</i>, 8 h after dawn for <i>Ehd3</i>, <i>OsMADS50</i>, <i>OsMADS51</i> and <i>DTH8</i> and immediately after dusk for <i>OsGI</i> and <i>Hd1</i> from 28 d-old (SDs) and 35 d-old (LDs) plants. The rice <i>Ubiquitin-1</i> (<i>UBQ</i>) gene was used as the internal control. Values are shown as mean±s.d (standard deviations) of three independent experiments and two biological replicates.