Reduced Cellular Proliferation in the <i>Irf6</i>−/− Tongue.

<p>Coronal sections through anterior, middle and posterior tongue of control and <i>Irf6</i>−/− mice at E13.5, E14.5, and E15.5 performed in triplicate. Using phosphohistone H3 (PH3) to label proliferative cells we identified PH3 reductions in E13.5 <i>Irf6</i>−/− anterior and posterior tongue (B, D) compared to control (A, C) that was significant (M). In the E14.5 mice tongues there was a dramatic decrease in PH3 labeled cells in the control tongue (E, G) compared to <i>Irf6</i>−/− tongues (F, H) that was significant (N). At E15.5 there continued to be reduced cellular proliferation in the <i>Irf6</i>−/− tongue (J, L) compared to controls (I, K) that was significant (Q). Statistical significance was determined using a student t test and P<0.05 used to determined significance (*).