Novel identification of AR target miRNAs.

<p><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0056592#pone-0056592-g003" target="_blank">Figure 3A</a>. RS distribution of androgen-responsive miRNAs. miRNA numbers according to different RS values are presented, the dashed line denotes the threshold for identifying AR candidate primary target miRNAs. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0056592#pone-0056592-g003" target="_blank">Figure 3B</a>. RT-PCR analysis for identified AR candidate target miRNAs. Fold change of DHT-treated LNCaP cells over control samples was presented with significance assessment (in this study, *: p<0.05; **: p<0.01; ***: p<0.001). Fold change in control samples was deemed as 1 for all RT-PCR analyses in this study. This figure is the RT-PCR analysis of miRNAs at 40 min of DHT stimulation. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0056592#pone-0056592-g003" target="_blank">Figure 3C</a>. Schematic diagram of miR-133b, miR-19a, and miR-27a’s ARE location in the 5′ and 3′ regions. The horizontal arrows indicate the approximate ARE locations. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0056592#pone-0056592-g003" target="_blank">Figure 3D</a>. ChIP assay of AR-binding on candidate targets of miR133b, miR19a, miR27a. ‘IgG’ serves as the negative control for ChIP assay.