Effect of curcumin and/or 5-FU on apoptosis in HCT116 and HCT116+ch3 colon cancer cells.

<p>HCT116 and HCT116+ch3 cells were treated with different concentrations of curcumin or 5-FU (0, 1, 5, 10 and 20 µM) or a combination of curcumin (5 µM) and 5-FU (0.1, 1, 2 and 3 µM) for 24 h. Monolayer cultures were stained with Hoechst 33258 (DAPI) to reveal apoptotic changes in the cell nuclei.