Inhibition of Cyclin D1 and Bcl-2 was responsible for the tumor suppressive effects of miR-195.

<p>(A), Inhibition of cell cycle progression by knockdown of Cyclin D1. SCC-15 and CAL27 cells were transfected with control RNA (Ctrl RNA) or Cyclin D1 siRNA as indicated. Cells were stained with propidium iodide (PI) at 48 h post-transfection and analyzed with FACS (<b>*</b><i>P</i><0.05, <b>**</b><i>P</i><0.01). (B), Promotion of apoptosis by knockdown of Bcl-2. SCC-15 and CAL27 cells were transfected with control RNA (Ctrl RNA) or Bcl-2 siRNA as indicated. Apoptotic cells were monitored with FACS after Annexin V and PI staining (<b>**</b><i>P</i><0.01).