Distribution of ApoE^null mice oral microbial samples positive for bacterial genomic DNA by PCR.

a<p>Indicate time points at which oral microbial samples were collected (2, 8, 14, and 16 weeks) following polymicrobial infection for determination of microbial colonization by species specific PCR analysis.</p>b<p>Indicate mice were infected for 8 alternate weeks with <i>P. gingivalis</i>, <i>T. denticola</i>, and <i>T. forsythia</i>. Samples were analyzed using appropriate specific PCR primers with positive and negative controls.</p>c<p>Oral microbial samples were collected from sham-infected control mice periodically and examined for <i>P. gingivalis</i>, <i>T. denticola</i>, and <i>T. forsythia</i> using bacteria specific primers and all mice were negative. - Indicate oral microbial samples negative by PCR analysis. <i>Pg</i>-indicate <i>P. gingivalis</i>; <i>Td</i>-indicate <i>T. denticola</i>; and <i>Tf</i>-indicate <i>T. forsythia</i>.