Induction of Meis1a/b expressions during neural differentiation of RA-primed P19 cells.

<p>Aggregated P19 cells were treated with 5×10⁻⁷ M RA for various times and the expression levels of Meis1a/b mRNAs and proteins were analyzed by RT-PCR and WB with the anti-Meis1 antibody, respectively. (<b>A</b>) Expression patterns of <i>Meis1a</i> and <i>Meis1b</i> mRNAs during neural differentiation. (<b>B</b>) Quantification of the expression levels of <i>Meis1a</i> and <i>Meis1b</i> mRNAs indicated in <b>A</b>. (<b>C</b>) Expression patterns of Meis1a and Meis1b proteins during neural differentiation. (<b>D</b>) Quantification of the expression levels of Meis1a and Meis1b proteins indicated in <b>C</b>. (<b>E</b>) Expression levels of <i>Meis1a/b</i> mRNAs in mouse fetal brain. Total RNAs from the developing brain were analyzed by Northern blotting. (<b>F</b>) Detailed expression patterns of <i>Meis1a</i> and <i>Oct4</i> mRNAs during neural differentiation. (<b>G</b>) Quantification of the expression levels of <i>Meis1a</i> and <i>Oct4</i> mRNAs indicated in <b>F</b>. (<b>H</b>) Detailed expression patterns of Meis1a and Oct4 proteins during neural differentiation. (<b>I</b>) Quantification of the expression levels of Meis1a and Oct4 proteins indicated in <b>H</b>. <i>Ribosomal large subunit protein P0</i> mRNA, β-actin and 28S ribosomal RNA were used as internal controls.