Effect of Meis1a on Sox2 and Pax6 expressions.

<p>(<b>A</b>) Expression patterns of Sox2 and Pax6 during neural differentiation. Aggregated P19 cells were treated with RA for various times and analyzed by WB with anti-Sox2 and Meis1 antibodies. (<b>B</b>) Quantification of expression levels of Sox2 and Pax6 as shown in <b>A</b>. (<b>C</b> and <b>D</b>) Stimulation of <i>Sox2</i> and <i>Pax6</i> mRNA and protein expressions by ectopic expression of Meis1a. Monolayer-cultured P19 cells were transfected with various amounts of pcDNA3-EF1-α-<i>Meis1a</i> and after 12 h, <i>Sox2</i> and <i>Pax6</i> mRNAs and proteins were analyzed by RT-PCR (<b>C</b>) and WB (<b>D</b>), respectively.