KLK4-expressing SKOV-3 cells are less migratory as 2D monolayers.

<p><b>A.</b> Western blotting with anti-V5 antibody shows KLK4 expression in the conditioned media (CM) and whole cell lysate (WCL) from stable KLK4 tranfectants (KLK4-1, KLK4-2 and KLK-3; lanes 1–3), vector (Vec-1, Vec-2 and Vec-3; lanes 4–6) and native SKOV-3 (lane 7) cells, and transiently expressed wildtype KLK4, mutant-KLK4S207A (KLK4S/A), vector and mock transfectants (lanes 8–11); GAPDH was used as a loading control for WCL. <b>B.</b> Western blotting with anti-KLK4 antibody shows relative levels of KLK4 protein in WCL of KLK4-1, KLK4-3 clones and OVCA432, and 10 ng of recombinant (r)KLK4 protein. <b>C.</b> IF microscopy with anti-V5/KLK4-N terminal antibodies (green) and phalloidin (red) in KLK4-1, Vec-1 clones, native SKOV-3 or negative control (IgG). Scale bar, 20 µm. <b>D.</b> Transwell migration assays with KLK4-1, KLK4-2, KLK4-3, Vec-1, Vec-2 and native SKOV-3 cells; n = 3, mean ± SEM, *P<0.05.